Multi-generational decisions in single cell biology
Sean Bendall, PhD
Associate Professor, Department of Pathology
Friday, April 19, 2024
11:00am - 12:00pm
James H. Clark Center, Room S360, 3rd floor next to the Coffee Shop
Zoom link
Abstract
Single cell and spatial proteomics, starting with the earliest low parameter fluorescent cytometry and microscopy experiments, helped define the major cell subsets and architecture of human tissues as we understand them today. Now, a novel combination of elemental mass spectrometry with single cell analysis (mass cytometry – CyTOF, Science 2011) and nanometer-scale imaging (multiplexed ionbeam imaging – MIBI, Nature Med. 2014, Cell 2018, Science Adv., 2019) offers routine, simultaneous quantification of > 40 proteomic features without fluorescent agents or interference from spectral overlap and autofluorescence using heavy metal isotopes as reporters. With this, we have reached new levels of understanding in tissue immune organization, combined with novel single-cell visualization and analysis methods. By identifying new cell populations, regulatory relationships, and structural rulesets we have identified numerous clinically predictive features underlying human disease.